Affinity chromatography is a separation process used to purify molecules or a group of molecules that are in a biochemical mixture. It employs two phases; a stationary phase and a mobile phase.

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

LCGC International spoke to John Cashman of Sartorius about the application and TFF and how it is being improved to be more environmentally sustainable.