Although 5'-3' nuclease oligoprobe (TaqMan assay) is the most widely used format owing to its commercial utility and higher specificity as a result of sequence-specific hybridization of the probe, the ...
Takara Bio has obtained a nonexclusive license from Roche Diagnostics for use of melting curve analysis technology and SYBR Green techniques in its kits and instruments for real-time PCR. The license ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
The TaqMan and SYBR Green I-based one-step real-time quantitative RT-PCR assays have potential utility for detection and quantification of CHIKV. These assay systems have been demonstrated to be rapid ...