Adapters consist of a core sequence (black) and an enzyme-specific sequence (red). The enzyme-specific sequence allows the ligation of the adapters to the resulting restriction fragments (green) ...

Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...

In the early 1970s, researchers reported that restriction enzymes cleave DNA at specific sites, producing individual fragments that are distinguishable using gel electrophoresis. 1,2 This timely ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...