Super-resolution microscopy - Wikipedia
WEBLearn about the techniques and methods that break the diffraction limit of optical microscopy and achieve higher resolution. Find out the history, applications and examples of super-resolution microscopy, including …
Super-resolution microscopy: a brief history and new avenues
WEBFeb 14, 2022 · Super-resolution microscopy (SRM) is a fast-developing field that encompasses fluorescence imaging techniques with the capability to resolve objects below the classical diffraction limit of optical resolution.
Super-resolution microscopy demystified - Nature Cell Biology
WEBJan 2, 2019 · In this Review, Schermelleh et al. give an overview of current super-resolution microscopy techniques and provide guidance on how best to use them to foster biological discovery.
What is Super-Resolution Microscopy? STED, SIM and STORM …
WEBDec 17, 2019 · Learn how super-resolution microscopy (SRM) techniques overcome the diffraction limit of light and achieve higher resolution than conventional light microscopy. Compare STED, SIM and STORM methods and see examples of subcellular imaging.
Visualizing and discovering cellular structures with super ... - Science
WEBAug 31, 2018 · Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously invisible molecular details in biological systems.
The cell biologist's guide to super-resolution microscopy
WEBJun 11, 2020 · Learn about the principles, strengths and weaknesses of different super-resolution microscopy techniques that bypass the diffraction limit of light. Compare the resolution, speed, sample preparation and compatibility of each method using radar plots and example images.
Super-resolution microscopy at a glance - PMC - National Center …
WEBMay 5, 2011 · Super-resolution images are created by assembling all of the localized points. Both photoactivation localization microscopy (PALM) (Betzig et al., 2006) and fluorescent PALM (fPALM) (Hess et al., 2006) use genetically expressed photoactivatable probes to achieve the on–off states of the fluorophore.
Super-resolution microscopy - Nature
WEBJan 29, 2018 · Advances in image scanning microscopy move super-resolution imaging deeper into tissues with faster visualization and finer details.
World’s most powerful 3D super-resolution microscope arrives at …
WEBFeb 21, 2024 · Using hollow sphere or “bottle-beam” laser excitation technology, the new microscope allows researchers to determine protein stoichiometries, or how many of each protein type make up a given protein complex. It uses fewer photons, which means less …
Multiview confocal super-resolution microscopy | Nature
WEBNov 26, 2021 · We demonstrate these capabilities on more than 20 distinct fixed and live samples, including protein distributions in single cells; nuclei and developing neurons in Caenorhabditis elegans embryos,...
Skip to content