Solid-Phase Peptide Synthesis: The Merrifield Method - SlideServe
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Robert Bruce Merrifield - Alchetron, the free social encyclopedia
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SOLVED: The fluorenylmethoxycarbonyl group is used to protect the amino group of amino acids during the Merrifield solid phase peptide synthesis procedure. The group is then removed by treatment with piperidine. The mechanism of addition and removal is as follows:The amino acid under basic conditions reacts with Fmoc chloride followed by deprotonation to give a tetrahedral intermediate I. The collapse of the tetrahedral intermediate gives the Fmoc-aa. In the removal sequence, deprotonation of th
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